DEV02830 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. RT-PCR analysis. (A) Design of the RT-PCR analysis used to examine exon 3 skipping. Forward (r, 5′-GTGTGCCGCCTTCCTGATTCTG-3′) and reverse (R, 5′-TGGGAGAGGAGGGAGGAACA-3′) primers were designed for exons 2 and 6, respectively. In the case of exon 3 skipping, a PCR product of 0.4 kb would be amplified from Dlgh1^−/− mice (bottom). (B) Results of RT-PCR for Dlgh1^+/+ and Dlgh1^−/− mice. No PCR product of 0.4 kb is detected, but a 1.3 kb product, representing a neo-inserted mRNA, is amplified from Dlgh1^−/− mice. M, molecular-weight marker DNA.

• Supplemental Figure 2 -

  Fig. S2. Sizes of Dlgh1^−/− mice. (A-D) Crown-to-rump (CRL) length of embryos at E18.5 (A) and E12.5 (D), and longitudinal lengths of the metanephric mesenchyme at E10.5 (B) and E11.5 (C) that are positive for Pax2 immunostaining. (E,F) The metanephric (E) and ureteric (F) length relative to the CRL at E12.5. Values are absolute (A,D) or are expressed as a percentage of the value for Dlgh1^+/+ mice (B,C,E,F). All data are indicated as mean±s.d. P values are indicated when there is a significant difference between the mean values of two groups.