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Fig. 7. Expression patterns of Dlgh1 and Ecadherin in embryos.
(A,B) Expression patterns of Dlgh1 (A) and Pax2 (B) in
Dlgh1+/+ mice at E11.5. Dlgh1 is expressed not only in the
Wolffian duct and ureter, which are Pax2-positive, but also in the epithelial
cells of the hindgut, which are Pax2-negative. In addition, mesenchymal cells
within the metanephric blastema are also weakly Dlgh1-positive. (C-J)
Subcellular distributions of Dlgh1 (C,G), E-cadherin (D,H, red in E,F,I,J),
occludin (green in E,I) and ZO-1 (green in F,J) in the ureteric epithelium of
Dlgh1+/+ (C-F) and Dlgh1-/- (G-J) mice
at E14.5 (C,D,G,H) and E15.5 (E,F,I,J). C,D and G,H represent each color of
the same sections, respectively. In Dlgh1+/+ mice, Dlgh1
is accumulated at the basolateral region of the ureteric epithelium (C),
whereas no significant Dlgh1-positive signals are detected in
Dlgh1-/- mice (G). The subcellular distributions of
E-cadherin, occludin and ZO-1 in Dlgh1-/- mice are similar
to those in Dlgh1+/+ mice; staining of E-cadherin in the
basement membrane of frozen sections (E,F,I,J) tended to be more intense than
that in paraffin sections (C,D,G,H). hg, hindgut; mn, metanephros; ur, ureter;
Wd, Wolffian duct. Scale bars: 100 µm in A,B; 10 µm in C-J.
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