DEV02843 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. The allantois in Mrj^−/− conceptuses is initially expanded but gradually contracts back to become a compact ‘bud’ after E9.0. (A) The allantoises of three representative Mrj^−/− embryos at E9.0, E9.25 and E9.5 (left to right). Each box includes an allantois and indicates the regions of higher magnification below (B-D). (B-D) Higher-magnifications of E9.0 (B), E9.25 (C) and E9.5 (D) allantoises (broken lines). Scale bars: 0.5 mm.

• Supplemental Figure 2 -

  Fig. S2. Placental hemorrhages reminiscent of K18/K19 double-knockout embryos form in a minority of Mrj-null conceptuses. Mrj-deficient conceptuses at E9.5 with placental hemorrhages (white arrowhead) in the trophoblast giant cell layer (A) or chorion (B). Boxed region in left panels represents higher-magnification in right panels. Black arrowhead indicates an allantois that has not attached to the chorion (broken line in A). Scale bars: 1.0 mm. ch, chorion; ys, yolk sac.