|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Mrj-/- embryos fail to undergo chorioallantoic
attachment because of defects within the chorionic trophoblast layer.
(A,B) Expression of the ß-galactosidase (ß-gal; A;
blue) and Mrj (B; red) proteins in E8.25 chorions. (C) Aggregation of a
diploid Mrj-/- embryo (blue) with a wild-type tetraploid
embryo expressing the Egfp transgene (green) results in a genetically
mosaic chorionic trophoblast, ectoplacental cone and trophoblast giant cell
layer (Rossant and Cross,
2001). The chorionic mesothelium, allantois and embryo proper are
almost exclusively composed of Mrj-deficient diploid cells.
(D,E) Rescued chorioallantoic attachment in an
Egfp:Mrj-/- chimeric conceptus at E9.5. (E)
Higher-magnification of boxed region in D. (F-H) Initiation of
branching morphogenesis in the placental labyrinth layer (arrowheads) at E9.5
in Egfp:Mrj+/+ (F) and
Egfp:Mrj-/- (H) chimeric placentas, but not in a
Mrj-/- chorion (G), which remains flat. al, allantois; ch,
chorion; em, embryo; me, chorionic mesothelial layer; tr, chorionic
trophoblast layer. Scale bars: 10 µm in A,B,F-H; 1 mm in D; 0.5 mm in
E.
|
