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Fig. 3. Keratin inclusion bodies disrupt cell-cell adhesion and disrupt the
organization of Mrj-deficient chorionic trophoblast cells.
(A) Mrj expression (red) in a wild-type chorion at E8.25.
(B,C) K18 expression (red) in wild-type (B; +/+) and
Mrj-deficient (C; -/-) trophoblast stem (TS) cells. DNA is blue.
(D) Ultrastructure of a keratin inclusion body (arrowheads) within a
Mrj-/- chorionic trophoblast cell at E8.25. (E-H)
K18 expression (red) in wild-type (E,F) and Mrj-/- (G,H)
chorionic trophoblast cells at E8.25. (F,H) Higher-magnifications of boxed
regions in E and G, respectively. (I-L) Morphology of Mrj
mutant chorionic trophoblast cells in vivo (J) and in vitro (L) in contrast to
wild-type cells (I,K). (M-P) Desmoplakin (Dp) expression (red) in
wild-type (M,N) and Mrj-/- (O,P) chorionic trophoblast
cells at E8.25. (N,P) Higher-magnifications of boxed regions in M and O,
respectively. (Q,R) Desmoplakin expression (green) in wild-type
(Q) and Mrj-/- (R) TS cells. (S,T) Electron
micrographs of desmosomes (arrowheads) in wild-type (S) and
Mrj-/- (T) chorionic trophoblast cells at E8.25. ch,
chorion; DP, desmoplakin; EPC, ectoplacental cone; me, chorionic mesothelium;
TGCs, trophoblast giant cells; tr, chorionic trophoblast. Scale bars: 10 µm
in A-C,E-R; 0.5 µm in D,S,T.
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