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Fig. 2. Real-time RT-PCR analysis of transcripts selected from microarray
expression profiles. (A-J) Five categories of transcripts were
selected for real-time RT-PCR analysis: transcripts that were most
dramatically up-(A,F), or down-(B,G) regulated in mutant cumulus cells;
transcripts that were specifically involved in certain metabolic pathways,
e.g. sterol biosynthesis (C,H), bile acid biosynthesis (D,I), and glycolysis,
purine metabolism, pyrimidine metabolism, pentose phosphate, and fructose and
mannose metabolism (E,J). Black bars indicate expression levels detected by
microarray, white bars indicate levels detected by real-time RT-PCR. Four sets
of cumulus cell samples from each genotype were used for real-time PCR
analysis and data are presented as mean ± s.e.m. of fold changes.
*P<0.05; DM vs WT, or Bmp15-/- vs
WT.
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