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Figure 4


Fig. 4. Effect of WT oocytes on expression of selected transcripts in WT OOX cumulus cells. WT COCs, OOX cumulus cells, and OOX cumulus cells + oocytes [two fully grown oocytes (FGO)/µl] were cultured for 20 hours, and expression of selected transcripts was detected by real-time RT-PCR using Rpl19 mRNA as internal control. (A) Transcripts upregulated in cumulus cells of both mutants. (B) Transcripts downregulated in cumulus cells of both mutants. (C) Transcripts encoding enzymes for cholesterol biosynthesis. (D) Transcripts involved in bile acid biosynthesis pathway. (E) Transcripts involved in other metabolic pathways: glycolysis, purine metabolism, pyrimidine metabolism, pentose phosphate, fructose and mannose metabolism, and inositol metabolism. Experiments were repeated three times. Data are presented as mean of the relative fold change in mRNA expression compared with COC group (control) ± s.e.m. Bars indicated with different letters are significantly different, P<0.05.





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