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Fig. 7. Comparison of levels of cholesterol synthesized in oocytes and/or
cumulus cells under various experimental conditions. (A) Comparison
of levels of cholesterol synthesized in WT cumulus-cell-enclosed oocytes
(CEOs) and denuded oocytes (DOs). The left panel is a representative TLC image
showing levels of [14C]cholesterol (indicated by arrow) production
in CEO and DOs. The right panel is the quantitative comparison of
[14C]cholesterol levels in CEOs and DOs. (B) Comparison of
levels of cholesterol synthesized in oocytes of WT, DM cumulus-oocyte
complexes (COCs), and DM COCs that were co-cultured with WT fully grown
oocytes (FGOs). The left panel is a representative TLC image showing levels of
[14C]cholesterol (indicated by arrow) produced by oocytes in each
group. The right panel is the quantitative comparison of
[14C]cholesterol levels in oocytes of each group. (C)
Comparison of levels of cholesterol synthesized in cumulus cells of WT, DM
COCs, and DM COCs that were co-cultured with WT FGOs. The left panel is a
representative TLC image showing levels of [14C]cholesterol
(indicated by arrow) produced by cumulus in each group. The right panel is the
quantitative comparison of [14C]cholesterol levels produced by
cumulus cells in each group. All experiments were repeated four times. Data
are presented as mean of the relative fold change in
[14C]cholesterol levels (levels in controls, i.e., CEO in A, WT in
B and C,=1) ± s.e.m. Bars indicated with different letters are
significantly different (P<0.05) using ANOVA and Tukey's HSD test.
The asterisk indicates significant difference by Student's t-test
(P<0.05).
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