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Figure 6


Fig. 6. The PTE is able to regulate expression of an ectopic promoter. In the W-5-EZ construct (top diagram), the IAB5 enhancer is located between the white and even-skipped (eve) promoters. In this configuration, IAB5 is able to drive expression from white (A) and eve-lacZ (B). Insertion of the 255 bp PTE sequence adjacent to the eve-lacZ promoter in the W-5-PTE-EZ construct (bottom diagram) resulted in strong expression of lacZ (D) and an absence of expression of white (C), indicating that IAB5 is now exclusively activating the eve-lacZ promoter.





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