|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. WLE3 mediates apical localization of wg transcripts.
(A) Fluorescent deletion constructs of wg 3'UTR RNA were
injected into D. melanogaster embryos. Localization efficiency was
categorized based on the apical:basal ratio of fluorescent RNA signal as
strong (++, ratio 
1.5), weak (+, 1.5>ratio1.0) or inactive (-,
ratio <1.0). The typical activity of each construct is indicated at the
right (* constructs typically inactive but occasionally localized).
WLE3 spans nucleotides 518-570, WLE1 57-183, and WLE2 661-776. (B-D)
Representative images of embryos injected with fluorescently labelled
full-length wg 3'UTR (B), deleted for WLE3 (C) or WLE3 dimer
(D). (E-G) Confocal images of stage 11
patched-GAL4::UAS-lacZ embryos containing different regions
of the wg 3'UTR fused to lacZ ORF:
WLE1-WLE2 (E); WLE1-WLE2-WLE3 (F); WLE3
(G). lacZ reporter transcripts (green) were detected by FISH. Nuclei
are red and membranes blue. Scale bars: 10 µm.
|
