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Fig. 4. Importance of sequence and base-pairing at conserved base-pair
positions. (A-C) Diagrams of D. melanogaster WLE3 (A) or
just the WLE3 distal stem (B,C). Bases targeted by each mutation are boxed in
the wild-type diagram, with arrows pointing to mutant sequences shown to
either side (mutant numbers/names are indicated at the bottom). The shading
indicates activity: white text on black, no activity (all -); white text on
dark grey, weak activity (mostly -); black text on light grey, moderate
activity (+); black text on white, full activity (++). To the far right of
each diagram are representative images of injected embryos. Scale bars: 10
µm. Base transversions are depicted at the immediate left or right of WLE3,
and compensatory mutations further right.
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