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Figure 3


Fig. 3. Cre expression in NG2creBAC:Z/EG double tg mice. Coronal sections (50 µm) through dorsal and ventral cortex and corpus callosum from a P14 double tg mouse were double labeled with rabbit anti-Cre (red in B,D,F,H,J,L) and guinea pig anti-NG2 (blue in C,D,G,H,K,L) antibodies. (A-D) In dorsal cortex (DC) Cre is expressed in NG2+ cells with EGFP fluorescence (arrows). (E-H) In ventral cortex (VC) Cre is expressed in NG2+ cells with EGFP fluorescence (arrowheads), but not in EGFP+ protoplasmic astrocytes, which are devoid of NG2 (arrows). (I-L) In the corpus callosum (CC) Cre is expressed in NG2+ cells with EGFP fluorescence (arrowheads), but not in EGFP+NG2- oligodendrocytes with large cell bodies and few short and thick processes (arrows). Cre is only present in NG2+ cells and not in EGFP-expressing astrocytes or oligodendrocytes in NG2creBAC:Z/EG double tg mouse. Scale bars: 20 µm.





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