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Fig. 5. EGFP+ astrocytes in ventral but not dorsal cortex of P14 NG2CreBAC:Z/EG
tg mice. Coronal sections (50 µm) through dorsal and ventral cortex and
corpus callosum from P14 double tg mice were double or triple labeled with
rabbit anti-EGFP (green in A,C,D,F,G,J,K,N,O,Q), mouse anti-GFAP (red in
B,C,P,Q), mouse anti-S100β (red in E,F; blue in I,J,M,N), and guinea pig
anti-NG2 (red in H,J,L,N) antibodies. (A-F) High magnification of EGFP+
astrocytes in ventral cortex. EGFP+ cells that have the typical morphology of
protoplasmic astrocytes with bushy processes are immunoreactive for GFAP (A-C)
or S100β (D-F). (G-J) EGFP+ cells in dorsal cortex are
immunoreactive for NG2. S100β+ protoplasmic astrocytes in dorsal cortex
are negative for NG2 and EGFP. (K-N) EGFP+ cells in ventral cortex
extending bushy processes characteristic of protoplasmic astrocytes are also
immunoreactive for S100β (thin arrows in M and N). EGFP+ NG2 cells are
positive for NG2 but devoid of S100β (thick arrows). Some S100β+NG2-
cells do not express EGFP (arrowheads). (O-Q) None of the EGFP+ cells
in the corpus callosum express GFAP. Yellow areas represent overlap of GFAP
and APC from adjacent cells, but the two antigens are never present in the
same cells. Scale bars: 20 µm.
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