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Fig. 7. Distribution of EGFP+ cells in embryonic forebrain of NG2creBAC:Z/EG
double tg mice. Coronal sections (20 µm) through the forebrain of an
E18 double tg mouse were triple labeled with mouse anti-GFP (green in
B,C,E,F), rabbit anti-NG2 (blue in B,C,E,F), and rabbit anti-GLAST (red in
B,C,D,E,F) antibodies. Dorsal is top, medial is left in B and right in C-F.
(A) The drawings represent E18 posterior (left) and anterior (right)
forebrains. The boxed areas indicate locations of the images in B-F. LV,
lateral ventricle. (B) A coronal section through the ventral subpial
region of anterior forebrain. Pial surface is lower left. The majority of
EGFP+ cells in anterior ventral forebrain express NG2 (arrows). A small number
of EGFP+ cells express GLAST and are NG2- (arrowhead). (C) Ventral
ventricular zone of posterior forebrain showing the ventral tip of the
posterior horn of the lateral ventricle. EGFP is not expressed in GLAST+
radial glia extending from the ventral germinal zone. EGFP and NG2 expression
in the SVZ is restricted to vascular cells. (D) A higher magnification
of the image shown in C stained for GLAST and DAPI. The cells bodies of radial
glia in the ventricular zone are labeled by GLAST and are negative for GFP.
Strong EGFP expression seen in the lateral ventricle in C and D is in the
choroid plexus. (E) A region immediately ventral to the ventral tip of
the SVZ underlying the ventral VZ. All the EGFP+ cells in this region express
NG2. GLAST+ radial glial fibers are EGFP-negative. EGFP+NG2+ cells in the
parenchyma have long slender processes. (F) A region ventral to E,
adjacent to the ventral pial surface. Clusters of EGFP+ cells localized close
to the ventral pial surface consist of two cell types: EGFP+NG2+ cells with
long slender processes (arrows) and EGFP+GLAST+ cells (arrowheads) with little
or no NG2. Some of the latter EGFP+GLAST+ cells have bushy processes
resembling immature protoplasmic astrocytes (cell with a star, shown in green
channel in inset). Scale bars: 20 µm.
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