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Figure 2


Fig. 2. Generation of Hoxc10 and Hoxd10 knockout alleles. (A) Schematic showing the wild-type (WT) genomic loci for Hoxc10 and Hoxd10, as well as targeted alleles for Hoxc10 and Hoxd10. (B) Southern blot analysis of DNA from ES cell clones for Hoxc10RFP and Hoxd10hrGFP. For Hoxc10RFP, ScaI digestion and a 3' flanking probe were used. The WT band is 11.5 kb and the correctly targeted band is 5.8 kb. For Hoxd10hrGFP, EcoRI digestion and a 3' flanking probe were used. The corresponding WT band is 12.4 kb, and the mutant band is 9.6 kb. (C) A typical hindlimb posture for Hoxc10-/-/Hoxd10-/- mutants and WT control.





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