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Figure 5


Fig. 5. Expression patterns of Raldh2, Pea3 and ER81 are altered in Hoxc10-/-/Hoxd10-/- double-mutant embryos. (A-C') In situ hybridization of Raldh2, Pea3 and ER81 in (A-C) whole-mount spinal cord (ventral view, rostral is to the top) and (A'-C') cross-sections through ventral spinal cord (outlined in black), as indicated in the drawing; lateral is to the left in each panel. D, dorsal root ganglion, outlined in white. Note that in Hoxc10-/-/Hoxd10-/- mutant embryos, Raldh2 and Pea3 expression was restricted to segments L3-L5, being absent from segments L1 and L2. By contrast, ER81 expression extends from thoracic segments into segments L1 and L2, but is lost from segments L3 and L4 in mutants. Scale bar: 100 µm for A'-C'.





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