DEV010363 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Analysis of PCNA expression in ventricular CMs during growth and size maintenance. (A) Ventricles from SG, RG and MS animals stained for Mef2 expression, to indicate CMs (red) and PCNA expression (green). The CM PCNA index was much greater in RG ventricles (arrowheads in insets). Nuclei are labeled with DAPI (blue). (B) Quantification of CM PCNA index. The average PCNA expression index per animal is plotted (*P<0.005, t-test, significantly different from SG; **P<0.005, t-test, significantly different from SG and RG). Numbers above the error bars indicate PCNA-positive CMs and total CMs counted from all animals combined. Six animals per group were quantified.

• Supplemental Figure S2 -

  Fig. S2. Rare TUNEL-positive CMs in the adult zebrafish ventricle. A single TUNEL-positive CM nucleus (arrowhead) in a ventricular section from a 6-month-old zebrafish, indicative of CM turnover. Scale bar: 100 µm.

• Supplemental Figure S3 -

  Fig. S3. hsp70:dn-fgfr1 animals display reduced mass increases during rapid growth. Fourteen days of RG conditions increase animal mass in both wild-type and transgenic zebrafish, but this increase is reduced in transgenics (*P<< 0.001, t-test, significantly different from Day 0; **P<0.005, t-test, significantly different from Day 0 and from wild-type Day 14; n=10). The Day 0 measurement represents pooled results of 35 transgenics and wild types.