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Fig. 3. Cardiogenic markers are induced during cardiac homeostasis.
(A) hand2 in situ hybridization of ventricular sections.
hand2 is weakly expressed in MS hearts, mildly expressed in SG hearts
and more strongly expressed in RG hearts similar to regenerating hearts 7 days
post-resection (arrowheads show foci of strong expression). (B)
Sections from SG, RG and MS ventricles of cmlc2:nRFP zebrafish,
stained for DsRed immunoreactivity. RG ventricles show many more
RFPcyto cells, a marker that suggests recent CM differentiation
events (arrowheads in insets). (C) Real-time Q-PCR analysis of
hand2 expression from whole ventricles of SG, RG and MS hearts,
relative to expression in ventricles 7 days after apical resection.
hand2 expression is similar in ventricles from RG fish to that in
regenerating ventricles (1.0, represented by dashed line) and greater than in
SG and MS ventricles. (D) Quantification of RFPcyto cells in
SG, RG and MS groups. The average RFPcyto index per animal is
plotted. Numbers above the error bars indicate RFPcyto CMs over the
total CMs counted from all animals combined. Numbers below group abbreviations
indicate the number of animals/ventricles per group
(*P<0.01, t-test, significantly different from
SG; **P<0.005, t-test, significantly different
from SG and RG). Scale bars: 100 µm.
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