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Figure 4


Fig. 4. MEKK1 is required for efficient transmission of the activin B signals to JNK. (A) M1+/{Delta}KDJ1-/- and M1+/{Delta}KDJ2-/- keratinocytes were either untreated (c) or treated with TGF{alpha} (T{alpha}, 10 ng/ml), TGFβ1 (Tβ, 10 ng/ml) or activin B (Act, 5 ng/ml) for 10 minutes, and cell lysates were analyzed for phosphorylated and total JNK and c-Jun, phospho-ERK and phospho-Smad2 by western blotting. Similar results were obtained from at least two independent experiments. The relative ratio of (B) p-JNK/total JNK and (C) p-c-Jun/total c-Jun in the Jnk1-null (J1-/-) and M1+/{Delta}KDJ1-/- keratinocytes either untreated (control) or treated with TGFβ and activin B were quantified by chemiluminescence imaging analyses and represented graphically. The levels in untreated cells were used as a standard for quantification. Similar results were obtained from at least three independent experiments.





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