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Fig. 2. Strong Uba1 alleles cause non-autonomous overgrowth. (A) Control mosaics: ey-FLP-induced clones in wild-type background. This phenotype is identical at 25°C and 29°C (shown is 25°C). Genotype: ey-FLP; FRT42D+/FRT42D P[w⁺]. (B,C) Clones of weak Uba1 mutants (phenotypically white^-) develop normally. Genotype: ey-FLP; FRT42D Uba1^H33 and Uba1^H42/FRT42D P[w⁺], respectively. (D) Eye mosaics of the strong Uba1^s3484 allele are overgrown with bulges (arrow). Genotype: ey-FLP;FRT42D Uba1^s3484/FRT42D P[w⁺]. This phenotype was obtained at 25°C, and is similar at 29°C. (E) Eye mosaic of the ts allele Uba1^H33 at 29°C. Uba1^H33 clones, which would be phenotypically white^-, are not detectable. The eyes appear rough, irregular and are often enlarged compared to wild-type eyes. Identical results were obtained for Uba1^D6. Genotype as in B. (F) Eye mosaic of the weak Uba1^H42 allele at 29°C. This allele does not behave as a ts allele. Phenotype and genotype are identical to those at 25°C (C). (G) Mosaic head of an Uba1^D6 animal raised at 29°C. The left half of the Uba1^H33 mosaic head is larger than the right half, suggesting that the overgrowth phenotype depends on the number and location of the Uba1 clones. Genotype as in B. (H,I) Eye-antennal imaginal discs of wild-type (H) and Uba1^D6 (I) mosaic animals raised at 29°C. These discs were labeled with Elav antibody which labels photoreceptor neurons to identify discs of comparable age. Photoreceptor differentiation does occur in strong Uba1 clones, but is weakly delayed (see also Fig. S1 in the supplementary material). Genotype as in A and B, respectively. Size bar: 100 µm.

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