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Figure 3


Fig. 3. Uba1 mutant clones affect ubiquitin conjugation. (A-D) The left panels indicate the positions of the Uba1 clones by absence of GFP, the middle panels show the experiment and the right panels are the merged images of left and middle panels. Genotype in all panels: ey-FLP; FRT42D Uba1/FRT42D P[ubi-GFP]. The allele is indicated in the panels. (A-C) Uba1 mutant discs were labeled with the FK2 antibody which specifically recognizes mono- and poly-ubiquitinated conjugates, but not unconjugated ubiquitin (Fujimuro et al., 1994). The strong alleles Uba1s3484 (A) and Uba1D6 (B, incubated at 29°C) significantly reduce FK2 immunoreactivity (see arrows). Clones of the weak allele Uba1H42 (C) mildly reduce FK2 immunoreactivity (arrows). (D) Uba1D6 mosaics incubated at 29°C. The overall protein levels of ubiquitin are unchanged in Uba1 mutant clones.





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