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Fig. 7. Notch signaling induces non-autonomous overgrowth in Uba1 mosaics. All discs were incubated at 30°C, except C which was incubated at 25°C. (A) Uba1^H33 mosaic eye discs labeled with Notch antibody C17.9C6 which was raised against the intracellular domain of Notch. Arrows indicate increase of Notch protein in Uba1 clones. This is most prominent immediately anterior to the morphogenetic furrow (arrowhead). Genotype: ey-FLP; FRT Uba1^D6/FRT P[ubi-GFP]. (B,C) Notch activity as visualized by the Notch reporter E(spl)m8 2.61-lacZ is increased in Uba1^D6 clones at 30°C (B) and 25°C (C). Genotype: ey-FLP; E(spl)m8 2.61-lacZ FRT uba^D6/FRT P[ubi-GFP]. (D) pSTAT labeling is increased in a non-autonomous manner in wild-type tissue immediately adjacent to Uba1 clones (arrow). Genotype as in A. (E) Eyes of Uba1^D6 mosaic animals are overgrown with bulges (arrow). Genotype as in A. (F,G) Heterozygosity of Notch (F) and stat92 (G) dominantly suppresses the Uba1^D6 overgrowth phenotype. Genotype: Notch²⁶⁴^-39/ey-FLP; FRT42 Uba1^D6/FRT42 P[ubi-GFP] (F) and ey-FLP; FRT42 Uba1^D6/FRT42 P[ubi-GFP]; stat92^85c9 (G).

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