DEV010108 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression of genes encoding subunits of the CAF1 and Pc-G complexes. Based on data available online from The A. thaliana Expression Database (http://csbdb.mpimp-golm.mpg.de/csbdb/dbxp/ath/ath_xpmgq.html).

• Supplemental Figure S2 -

  Fig. S2. Localization of mutations in the four msi1 loss-of-function alleles used in our study. The details of the mutations associated with the new alleles msi1-3 and msi1-4 are shown. Arrowheads indicate the precise location of base-pair changes. Asterisks indicate stop codons. The sequence underlined was replicated and replaced the wild-type sequence as shown in bold in msi1-4.

• Supplemental Figure S3 -

  Fig. S3. Viability of pollen from msi1/+;qrt/qrt plants. Alexander staining of pollen from msi1/+;qrt/qrt at uni-nucleate stage (A), bicellular stage (B) and tricellular stage (C,D). Arrowhead indicates dead pollen grains. Scale bars: 10 µm.

• Supplemental Figure S4 -

  Fig. S4. Seed abortion caused by pollination with msi1-1/+. The errors bars correspond to the standard deviation observed in the population (n).

• Supplemental Figure S5 -

  Fig. S5. In vitro pollen germination of combination between mutations in members of the CAF1 complex. Three replicates were performed for each assay. In each replicate, 300 pollen grains were scored for pollen germination rates. Error bars correspond to standard errors. Germination condition is referred to by Boavida and McCormick (Boavida and McCormick, 2007).

  Boavida, L. C. and McCormick, S. (2007). Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana. Plant J. 52, 570-582.

• Supplemental Figure S6 -

  Fig. S6. Summary of the classes of abnormal pollen produced by msi1 mutants and their impact on fertilization.