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Figure 6


Fig. 6. Pollination of wild-type ovules with msi1 pollen leads to single fertilization events. (A) Wild-type Arabidopsis seed with an embryo and an endosperm, 2 days after pollination (2 DAP). (B) Seed with an endosperm but without an embryo, 3 DAP. At the embryo region, the egg cell remains unfertilized. (C) Seed with an embryo but without endosperm, 3 DAP. At the side of the embryo the nucleus of central cell that remains unfertilized is still observed. (D) Fully aborted ovule without embryo and endosperm, 3 DAP. The egg cell and the central cell remain unfertilized. (E) Expression of the fluorescent KS22 GFP endosperm marker in a wild-type seed at 3 DAP. (F) Expression of KS22 GFP in a seed containing only endosperm from a cross between wild-type ovules and msi1-2/+; KS22/KS22 pollen. (G) Confocal section of a seed containing only an embryo, produced by a cross between a wild-type ovule and msi1-2 pollen. The nucleus of the unfertilized central cell remains associated with the surface of the embryo. Feulgen staining marks brightly the DNA in nuclei as described previously (Garcia et al., 2003). The inset shows ten chromocentres at pre-prophase in the nucleus from the single embryo. The single embryo is diploid and thus originates from a fertilized egg cell. Scale bars: 100 µm in A-D; 50 µm in E,F; 10 µm in G. cc, central cell; ec, egg cell; em, embryo; en, endosperm.





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