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Figure 3


Fig. 3. The DFCs are specified and migrate to the tailbud in thapsigargin-treated embryos. sox17 expression at 80% epiboly in (A) wt and (B) thapsigargin-treated embryos. Enriched Ntl protein at 80% epiboly in the DFCs in (C) wt and (D) thapsigargin-treated embryos; arrowheads note the DFC region. Expression of sox17 at 4-5 somites in (E) wt and (F) XeC-treated embryos; asterisks indicate dispersed cells, arrows indicate DFC/KV region. Tg (Dusp6:d2EGFP) expression at 80% epiboly in (G) untreated and (H) thapsigargin-treated embryos; arrows indicate DFCs. EGFP expression at tailbud to 1-somite in (I) untreated and (J) thapsigargin-treated embryos and at 5 somites in (K) untreated and (L) thapsigargin-treated embryos. XeC, Xestospongin C; n, notochord.





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