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Fig. 3. Wnt3a and β-catenin regulate segment polarity and boundary formation by positioning the expression of segment determination genes. (A-F) Uncx4.1 expression in wild-type (A,B), T-Cre;Ctnnb1^flLOF/ (C,D), and T-Cre;Ctnnb1^flGOF/+ (E,F) 4-6 ss embryos. Note that B and D are ventral-posterior views, whereas F is an anteroventral view. The curved line depicts the extent of the expression domain along the AP axis. (G-J) Two-color WISH depicting Uncx4.1 (orange) and Tbx18 (purple) expression in wild-type (G), Wnt3a^-/- (H), T-Cre;Ctnnb1^flLOF/ (I), and T-Cre;Ctnnb1^flGOF/+ (J) 5-7 ss embryos. (K-R) Two-color WISH depicting Uncx4.1 (orange) and Mesp2 (purple, arrows) expression in wild-type (K,O), Wnt3a^-/- (L,P), T-Cre;Ctnnb1^flGOF/+ (M,Q), and Wnt3a^-/-; T-Cre;Ctnnb1^flGOF/+ (N,R) 10 ss embryos. O-R are high-power magnifications of dorsal (O,P) and ventral (Q,R) views. (S-V) Mesp2 expression in S-I (S) is posteriorized, relative to the node (line), in Wnt3a^-/- embryos (T), and nearly absent from T-Cre;Ctnnb1^flLOF/ 2-5 ss embryos (U). Mesp2 is anteriorized and expressed in multiple stripes (arrows) in T-Cre;Ctnnb1^flGOF/+ embryos (V). 3a, Wnt3a^-/-; LOF, loss of function; GOF, gain of function. Scale bars: 100 µm.

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