QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. MMP LOF mutants display inappropriate ISNb defasciculation. In each micrograph, two abdominal hemisegments of stage 17 dissected embryos stained with -FasII (A-D) or -GFP (I-L) to label the motor projections, are shown with anterior left and dorsal up. Below each image are schematics of the observed phenotypes with motor axons in brown (E-H) or black (M-P) and muscles represented by gray boxes. In addition to carrying mutations in the indicated MMP genes, embryos in I-L are heterozygous for both Hb9^Gal4 and UAS-mCD8GFP to visualize Hb9-positive axons with -GFP. (A,E,I,M) Wild-type embryos have tightly bundled axonal projections. (B,F,J,N) Mmp1^Q112*/Mmp1² mutant embryos exhibit moderate ISNb defasciculation. (C,G,K,O) ISNb-projecting axons in Mmp2^W307*/Mmp2^Df embryos are loosely bundled and frequently project aberrantly within the VLM field (arrowheads). (D,H,L,P) Mmp2^W307* Mmp1^Q112*/Mmp2^Df Mmp1² double mutants are indistinguishable from Mmp2 single mutants with frequent ectopic projections (arrowheads). Scale bar: 15 µm.

Return to article