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Figure 3


Fig. 3. wol encodes the Drosophila ALG5 UDP-glucose:dolichyl-phosphate glucosyltransferase. (A) The gene CG7870/ALG5 consists of four exons encoding a 326 amino acid protein (coding region shaded dark gray). The lesions in the wol1 and wol2 alleles are shown. A transgene extending from -474 to +2340 was able to rescue the lethality and fertility of the wol alleles. (B-D) Expression of wol/ALG5 in wild-type embryos (lateral views in B,C, and a dorsal view in D). In cellularizing embryos (B), maternal ALG5 RNA is ubiquitous. At stage 11, zygotic exression begins in salivary gland precursor cells (arrowhead, C). Strong expression in salivary glands and part of the proventriculus is detected in stage 16 embryos (D). (E) Western blot analysis of carboxypeptidase Y (CPY). CPY glycoforms lacking one (-1) or two (-2) oligosaccharide chains can be detected in a {Delta}alg5 mutant yeast strain. Transformation of yeast (pScALG5) or fly ALG5 (pDmALG5) cDNA restores CPY glycosylation, whereas fly ALG5 cDNAs with either the wol1 or wol2 mutations fail to do so.





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