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Figure 4


Fig. 4. The unfolded protein response (UPR) is triggered in wol embryos. (A) Splicing of xbp1 mRNA was examined by RT-PCR. In wild-type Drosophila embryos, only the unspliced 127 bp product was detected, whereas some spliced 104 bp xbp1 message was found in wol1 embryos. In untreated S2 cells, mostly unspliced product is present. Tunicamycin (tm) or DTT treatment shifts the ratio of spliced/unspliced in favor of the spliced product. (B) Increased eIF2{alpha} phosphorylation in wol embryos. Western blot analysis of phosphorylated and total levels of eIF2{alpha}. The ratio of phosphorylated/total eIF2{alpha} was calculated from two independent experiments.





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