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Fig. 8. Expression of the root apical meristem marker genes in the obe1
obe2 double mutant. (A,F) QC46 expression in the
central root cap of wild-type (A) and obe1 obe2 (F) seedlings.
(B,G) QC46 expression in wild-type (B) and obe1 obe2
(G) heart stage embryos. (C-E,H-R) In situ hybridization with
WOX5 (C,D,H,I), MP (E,J), PLT1 (K,L,O,P) and
SCR (M,N,Q,R) antisense probes hybridized to embryos from
self-fertilized OBE1/obe1 obe2/obe2 plant.
Longitudinal sections of in situ hybridization specimens are shown. (C,H)
Early globular stage embryos. (D,I,L,P) Late globular stage embryos. (E,J,N,R)
Heart stage embryos. (K,O) Eight-cell stage embryos. (M,Q) Globular stage
embryos. About one quarter of the embryos from self-fertilized
OBE1/obe1 obe2/obe2 plants failed to express
WOX5 (H,I), PLT1 (O,P) or SCR (Q,R), while the
remaining three-quarters of the embryos exhibited wild-type-like expression of
the above genes. By contrast, MP expression was detected in all
embryos (J). Scale bars: 25 µm in A-J,L-N,P-R; 10 µm in K,O.
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