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Fig. 5. Syn4 controls the persistence of NC migration. A sox10:egfp
zebrafish transgenic line was used to analyze NC migration in vivo.
(A-D,F-I) Time lapse of embryos at 16 hpf carried out for 4
hours, three frames (t=0.0 min, t=2.43 min and
t=3.55 min) are shown; first frame is also overlapped with a DIC
image. Dorsal to the left; anterior to the top. Arrow indicates an individual
cell as an example. (E,J) Trajectories of 4 hours of migration.
(A-E) Control MO; (F-J) syn4 MO. (K) Trajectories of cells
from different experiments. Blue, control MO; red, syn4 MO.
(L) Velocity of migration. (M) Persistence of migration.
(N) Angle of migration for each individual cell. Blue, control MO; red,
syn4 MO. (O,P) Rose plot showing the distribution of
angles of each time point during the 4 hours of migration. The area of each
bin represents the number of cells moving in that direction. (O) Control MO;
(P) syn4 MO. ***P<0.005.
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