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Fig. 1. MALS-3 is expressed by and localizes to the apical surface of NPCs.
(A-C) Immunostaining of E14 coronal sections of rat telencephalon with
antibodies against MALS-3 (green), ZO2, F-actin or β-catenin (red) reveal
the supra-apical localization of MALS-3 relative to ZO2 (A), β-catenin
(B) and F-actin (C). Nuclei are stained with SYTO-11 (blue). The en face view
in C shows the lumen of the ventricle at the bottom, then (from bottom to top)
passes through the apical-most ends of VZ cells and into the domain occupied
by adherens junctions, which appear as circles of F-actin staining.
(D-L) MALS-3 is initially localized diffusely in VZ cells (D-F), but
appears to adopt an apical localization at the onset of neurogenesis
(
E11.5, G-I), which is maintained throughout the rest of embryonic life
(J-L). Scale bar: 5 µm.
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