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Fig. 7. The mislocalization of MALS-3 disrupts polarity. (A-R)
Analyses of brains electroporated with FL_MALS-3/EGFP (A-C),
Myr_MALS-3 (E,F,H,I,K,L,N-R) or Myr_CRB3 (D,G,J,M) at E13.5
and sacrificed at E15.5 (D-O) or E18.5 (P-R). Brains electroporated with
FL-MALS-3 show no changes in the apical localization of CRB3 (A),
PALS1 (B) or aPKC
(C). Brains electroporated with Myr_CRB3 show
no change in the apical localization of ZO1 (red, D), PATJ (red, G), or PALS1
(red, J) in GFP-positive cells (green). (M) CRB protein (red) is apparent in
the basolateral regions of GFP-positive cells (green) following
electroporation with Myr_CRB3, as expected. Myr_MALS-3
electroporated, GFP-positive cells (green) show a loss of apical localization
of ZO1 (E,F), PATJ (H,I) and PALS1 (K,L). CRB staining (red) appears reduced
in some GFP-positive cells (N,O). At E18.5, Myr_MALS-3 electroporated
brains reveal misplaced cells in the lateral ventricles and breaks in the VZ,
and MALS immunostaining is lost at those breaks (P). Larger breaks and loss of
PALS1 staining (red) are also observed (Q). The ventricles are populated by
delaminated cells that are TuJ1-positive (R). Arrows point to GFP-positive
cells in the VZ that are not positive for ZO1 (E), PATJ (H), PALS1 (K), CRB
(N), MALS (P) and PALS1 (Q). Scale bar: 10 µm.
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