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Figure 5


Fig. 5. Partial rescue of the head and forebrain defects in the Dkk1-/- embryo with reduced Wnt3 gene dose. (A) Comparison of the morphology of the Dkk1+/+;Wnt3+/+, Dkk1-/-;Wnt3+/- and Dkk1-/-;Wnt3+/+ embryos showing the almost complete to partial restoration of head structures in the Dkk1-/-;Wnt3+/- embryo at E9.5 and E10.5. (B) Marker analysis of wild-type (Dkk1+/+;Wnt3+/+), `rescued' (Dkk1-/-;Wnt3+/-) and Dkk-null (Dkk1-/-;Wnt3+/+) embryos (E8.5: TopGal, Six3 and Fgf8; E9.5: En1 and Dlx5). The presence of TOPGal-free and Six3-expressing domains (arrows) show the restoration of forebrain tissue in the `rescued' embryos. Expression of Fgf8 is present in the commissural plate (arrow) of the rescued embryo but is weaker than the wild type, whereas the isthmus is restored to a normal position (asterisk) that is shifted rostrally in the Dkk1-/-;Wnt3+/+ embryo. En1 is restored to nearly wild-type pattern in the midbrain of the rescued embryo (asterisk). Dlx5 expression (arrows) reveals the improved development of the branchial arch in the rescued embryo.





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