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Fig. 4. Lateral plate mesoderm contributes to endothelial cells but not to SMCs
in the descending aorta at E11.5. (A,B) A single layer of
lacZ-expressing cells was detected in the aortic wall of
Hoxb6-cre/R26 reporter mice. The aorta was surrounded by large areas
of lacZ-negative cells in the anterior part of the descending aorta,
shown here in the lung bud region (A), and by four or five layers of
lacZ-negative cells in posterior parts, shown here in the gonadal
ridge region (B). (C) Triple staining against Acta2 (green), CD31 (red)
and lacZ (blue) showed that endothelial cells, but not SMCs,
expressed the R26 reporter gene. (D,E) Similarly,
double-staining against lacZ and Acta2 showed lacZ staining
in endothelial cells but not in SMCs (high-power magnification of boxed area).
(E) Cells that were double-labeled for lacZ and Acta2 were detected
in a discrete region in which lateral plate mesoderm-derived cells were still
found in proximity to the aorta (high-power magnification of boxed area).
Arrows indicate lacZ-negative SMCs; arrowheads,
lacZ-positive endothelial cells; asterisks, lacZ-positive
SMCs; ao, aorta; lb, lung bud; cv, cardinal vein; gr, gonadal ridge; pc,
peritoneal cavity. Scale bars: 25 µm in A-E; 10 µm in higher
magnification views.
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