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Fig. 5. Hes repress proneural proteins, but not E-protein expression.
(A-D) Electroporation of NICD induced Hes1 (A) and
Hes5 (B) expression. Misexpression of dnCSL downregulated
Hes1 and Hes5 (C,D). (E,F) Misexpression of
Hes5 efficiently attenuated Ngn2 expression (E) but had no effect on
E47 transcription (F). (G-I) Misexpression of Hes5 suppressed
Ngn2 (G) and Tuj1 expression (H,I). (J-O) Co-transfection of
Ngn2 and Hes5 promoted electroporated cells to differentiate
into post-mitotic neurons (J-L), whereas misexpression of Hes5 in combination
with E47 blocked neuronal differentiation (M-O). Embryos were analyzed 24
hours after electroporation. Black and white representation of G,H,J,K,M,N are
shown in Fig. S7 (see supplementary material). Data in I,L,O are represented
as percentage of electroporated cells expressing Tuj1, mean±s.e.m.
*P<0.05, **P<0.01, relative to
EGFP-transfected control cells, Student's t-test. Scale bar:
50 µm.
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