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Fig. 5. PIII injection reduces opV trigeminal placode induction in ovo, but does
not alter proliferation, cell death or total cell number. (A)
Vehicle or PIII solution was focally injected at st. 8 and placode induction
assessed at st. 10. PIII (1 µm) (n=6) resulted in 71% reduction of
Pax3 relative to controls (n=7). Concentrations of 2.5 and 5 µm
PIII caused 67% and 84% reduction (n=8, n=6), respectively.
Error bars are s.e.m. *P<0.001. (B) No
differences in phospho-histone H3+ were noted between control and 2.5 µm
PIII embryos (P>0.05; n=5 each). (C) TUNEL
staining revealed no difference in cell death between control and 2.5 µm
PIII-injected embryos (P>0.05; n=5 each). (D)
Total cell number as assayed by DAPI was not different between control and 2.5
µm PIII-injected embryos (P>0.05; n=5 each).
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