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Figure 7


Fig. 7. dnPDGFRβ blocks Pax3 induction. (A-C) Control empty pCIG vector co-expresses Pax3 and GFP. (D-F) dnPDGFRβ-pCIG vector dramatically reduces the expression of Pax3. (G) Quantification of Pax3/GFP+ shows a greater than 50% reduction with expression of dnPDGFRβ (*P=0.036). (H) Quantification of transfection (GFP/DAPI+ cells) efficiency shows no significant difference between control and dnPDGFRβ (P>0.35). Arrows indicate double-positive Pax3/GFP+ cells.





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