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Fig. 1. Expression pattern of β-catenin in the developing
testis. (A-L) β-Catenin (red)/AMH (green) double
immunostaining at different stages. Nuclei were counterstained with DAPI
(blue). (A,C,E,G,I,K) β-Catenin channel only and (B,D,F,H,J,L)
β-catenin and AMH channels. β-Catenin was detected in the germ cells
predominantly in the plasma membrane and cytoplasm at all stages. Sertoli
cells started to express β-catenin from E15.5, mainly on the Sertoli cell
membrane, but not in the nucleus (G-L). SC, Sertoli cell; GC, germ cell. Scale
bar: 20 µm.