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Figure 9


Fig. 9. Cell proliferation and apoptosis in Catnblox(e3)/+; AMH-Cretg/+ Sertoli cells. Immunostaining with proliferative marker BrdU (A,D) and PH3 (B,E) identified proliferating Sertoli cells of both control and mutant testes at E15.5 (arrowheads). (C,F) TUNEL staining identified no apoptotic cells in either control or mutant testes at E15.5; inset in C shows apoptotic cells in the regressing Müllerian duct as a positive control. Scale bar: 20 µm.





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