DEV016147 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Shh^−/− mutants largely lack Ptch1^lacZ DM activity at E11.5. (A) Sagittal sections of Ptch1^lacZ embryos demonstrate atrial lacZ staining confined to the DMP. (B) Shh^−/−; Ptch1^lacZ mutants lack this domain of atrial lacZ staining.

• Supplemental Figure S2 -

  Fig. S2. Shh^−/− mutants demonstrate abnormal AV cushion rightward expansion. E11.5 section analysis of Shh^−/− embryos indicates abnormal cushion distribution along the left-right axis of the heart. (A,B) AV cushion area calculated for consecutive sections in a representative wild type (A) and mutant (B) demonstrates that the inferior and superior cushions follow a similar distribution trend along the right-left axis in wild-type embryos, but in Shh^−/− embryos this trend is uncoupled. Vertical lines in A and B represent the histological landmark of the point of fusion between the superior AV cushion and the mesenchyme of the primary atrial septum. Although AV cushion distribution to the right and left of this midline feature is roughly equivalent in the wild-type embryo, more AV cushion tissue remains to the left of this point in the Shh^−/− mutant.