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Fig. 1. DM contributions to atrial septation are marked by
Mef2C-AHF-Cre. (A-F) Mef2C-AHF-Cre mediated
R26R expression. Whole-mount (A) and sagittal section (B) at E10.5
demonstrates DM expression leading into the atria. (C) At E11.5 DM expression
is continuous with the DMP in the atria. (D) Transverse E12.5 section
demonstrates interface between the DMP and AV cushion-derived CMM. (E,F)
Transverse sections at E14.5 show expression in DMP and PAS but not in AV
cushion derived tissues. (G,H) Comparison of
Mef2C-AHF-Cre; R26R transverse sections and MRM slices
enabled 3D MRM reconstruction of the heart. (G) Sample color-labeled
transverse slice demonstrating labeling scheme used for renderings. (H) 3D
reconstruction depicts heart from a dorsal-lateral perspective with AV
cushion-derived tissue labeled yellow and DMP labeled green. A, atria; Ao,
aorta; CMM, central mesenchymal mass; DM, dorsal mesocardium; DMP, dorsal
mesenchymal protrusion; IAVC, inferior atrioventricular cushion; L, lung; LA,
left atria; LBPa, left branch pulmonary artery; LV, left ventricle; MV, mitral
valve; OFT, outflow tract; PA, pharyngeal arch; PAS, primary atrial septum;
RA, right atria; RBPa, right branch pulmonary artery; RV, right ventricle;
SAVC, superior atrioventricular cushion; SM, splanchnic mesoderm; TV,
tricuspid valve.
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