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Figure 3


Fig. 3. AV cushion formation is normal in Shh-/- mutant embryos. Wild-type (A) and Shh-/- (B) E10.5 AV cushion sagittal sections stained with PECAM1 (blue) and nuclear marker (green) demonstrated no significant change in morphology or total mesenchyme per cushion (quantitated in C; wild type and Shh-/- n=4). Identical analysis at E11.5 found no significant change in total mesenchyme cells or cushion volume (D; wild type and Shh-/- n=3). A, atria; V, ventricle; IAVC, inferior atrioventricular cushion; SAVC, superior atrioventricular cushion.





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