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Fig. 6. Slug or Twist rescue the vascular defects provoked by Xc-Myc
knockdown. (A) The percentage of embryos that appeared normal
(dark-blue bars) or showed edema associated with hemorrhage (light-blue bars)
after injection of Xc-Myc morpholino alone or mixed with
Xc-Myc-Mut, wild-type Xc-Myc, Slug, Twist or
β-galactosidase mRNAs. (B) Representative images of the
results in A. showing the rescue of the Xc-Myc knockdown phenotype by Slug or
Twist. (C) One blastomere of a two-cell stage embryo was injected with
Slug morpholino. In situ hybridization at the neural tube stage using
a probe specific for Xc-Myc showed that knockdown of Slug indeed
affects Xc-Myc expression in the region corresponding to the neural
crest (i-iii). On the injected side (black arrowheads) an accumulation of
Xc-Myc-expressing cells is evident, while on the uninjected side cells
expressing Xc-Myc have started to migrate (red arrow). A deviation in the body
axis is also evident (ii,iii). Despite affecting Xc-Myc expression
during neurulation, knockdown of Slug in two-cell embryos is not
sufficient to cause the edema and hemorrhagic phenotype (iv,v), as observed
upon Xc-Myc knockdown using Xc-Myc morpholino.
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