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Figure 3


Fig. 3. Structure and expression of the Drosophila Mop protein. (A) Mop contains regions of homology to Bro1 and to tyrosine phosphatases. The positions of stop codons introduced by three mop mutant alleles are indicated. (B) Comparison of the Mop tyrosine phosphatase domain with the consensus sequences for the ten functional motifs defined for tyrosine phosphatases (boxed). Amino acids that differ from the consensus sequence are in red; the arrow indicates the predicted active site cysteine. (C-F) Third instar eye imaginal discs with clones positively marked by GFP expression (green in D,F) stained with anti-Elav (C,E, magenta in D,F). (C,D) mopT482 clones expressing a wild-type UAS-mop transgene; (E,F) mopT482 clones expressing a phosphatase-dead transgene (UAS-mopCS). Both transgenes fully rescue photoreceptor differentiation. (G-J) Third instar wing imaginal discs expressing aos-lacZ and ap-GAL4 and stained overnight with X-Gal. ap-GAL4 drives expression in the dorsal compartment (outlined in G). (G) Wild type; aos is weakly expressed in the wing vein primordia (bracket). (H) UAS-FlagMop; (I) UAS-EGFR{lambda}top; (J) UAS-FlagMop and UAS-EGFR{lambda}top. Mop overexpression gave very weak ectopic aos expression (arrow, H), but strongly enhanced the effect of activated EGFR.





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