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Figure 4


Fig. 4. Mop is an endosomal protein. (A) Drosophila S2R+ cells stained for endogenous Mop. (B,C) GFP-Rab7 fluorescence in live S2R+ cells treated with dsRNA targeting lacZ (B) or mop (C). Mop depletion causes enlargement of Rab7-containing endosomes. (D-I) Wing discs expressing UAS-FlagMop with ap-GAL4 and stained for Flag (magenta in D,E; green in G,I), coexpressed Rab5-GFP (green in D), Rab11 (green in E,F), or Dor (magenta in G,H). (J-L) S2R+ cells expressing UAS-FlagMop and UAS-GFPRab5 (J), UAS-GFPRab7 (K) or UAS-GFPRab11 (L) with Actin-GAL4. Flag staining is shown in magenta and GFP in green. Mop is present on vesicles that are adjacent to Rab5-containing vesicles (arrowheads in D,J), shows partial colocalization with Dor and Rab7 (arrowheads in G,K), and does not colocalize with Rab11. (M,N) Wing discs with mopT612 clones marked by the absence of GFP (green in N), stained with anti-Hrs (M, magenta in N). Hrs shows reduced levels and punctate localization (arrows, M) in mop mutant clones. (O) An eye disc with a clone of cells expressing UAS-FlagMop and UAS-EGFR{lambda}top, stained with anti-Flag (green) and anti-EGFR (magenta). Most of the activated EGFR is present in vesicles that do not contain Mop. Scale bars: 10 µm.





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