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Fig. 1. Subcellular localization of Gurken in follicle cells. Anterior of
the egg chamber is towards the left and dorsal side faces upwards.
(A-D) The localization of Gurken in the posterior follicle cells during
early oogenesis stages was dependent on shibire. Gurken was labeled
with antibody (green). The oocyte and follicle cell cortex were visualized by
phalloidin staining (red). Gurken was easily detected in the posterior
follicle cells around the oocyte at permissive temperature in
EQ1::shits egg chambers (A,B). These punctate signals were
significantly reduced at a non-permissive temperature (C,D). (E-H)
Gurken (red) colocalized with early endosomes (labeled with GFP-Rab5, E,F),
and late endosomes (labeled with GFP-Rab7, G,H) in posterior follicle cells.
Arrows indicate colocalization in the merged images (F',H').
(I-L) During middle oogenesis when Gurken (green) shows an asymmetrical
localization in the oocyte, little Gurken could be detected in dorsal follicle
cells. At stage 8 (I,J) and 9 (K,L), Gurken signals in dorsal follicle cells
were detected in about 17% of the egg chambers. Scale bars: 10 µm in A-I;
20 µm in J,K; 40 µm in L.
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