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Fig. 4. The degradation of HRP-Grk in follicle cells. (A) Schematic representation of our sectioning strategy. The data shown below were all taken from cross-section profiles of stage 9 egg chambers that included oocyte nucleus. (B) Micrograph of a 1 µm cryosection. N, oocyte nucleus; Oo, oocyte; FC, follicle cells. (C-G) Electron micrographs of stage 9 oocytes immunolabeled with anti-HRP (10 nm gold particles). The labeling density of HRP-Grk at TGNs (brackets) is higher on the dorsal side (C) than on the ventral side (D) of the egg. (E) Quantitation of HRP-Grk labeling per TGN unit in the oocyte. n=310. Among the organelles, the single-membrane delimited MVB/lysosome (arrows) exhibited the most concentrated labeling, both in the dorsal (F) and the ventral (G) follicular cells. M, mitochondria. (H-J) The MVB/lysosomes in follicle cells of late stage 9 OreR egg chamber. Electron micrographs of MVB/lysosomes in the dorsal (I) and lateral (J) follicular cells. (H) Semi-quantification of the volume density of MVB/lysosomes in follicular cells. The average density ratio (bar) in dorsal, and lateral follicle cells is 0.0060 and 0.0109, respectively. The difference between the dorsal and lateral follicle cells is meaningful in the nonparametric Wilcoxon test (two-tailed, P=0.007). (K) Semi-quantification of the volume density of MVB/lysosomes in follicle cells of late stage 9 grk^HF/grk^HF egg chambers. The average density ratios (bar) for dorsal and lateral are 0.0062 and 0.0065, respectively. Scale bars: 20 µm in B; 100 nm in C,D,F,G,I,J.

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