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Fig. 5. Overexpression of CblL altered Gurken distribution. Anterior of the
egg chamber is towards the left and dorsal side faces upwards. (A-C)
Gurken expression pattern (green) at late stage 9 egg chambers was altered
when Cbl was overexpressed. In wild-type egg chambers, Gurken accumulated in
the dorsal anterior corner (A). Slightly reduced expression of Gurken was
detected in egg chambers with overexpression of CblS driven by
GR1-Gal4 (B). Significantly reduced expression of total Gurken in egg
chamber in which CblL was overexpressed (C). (D) The percentage of late
stage 9 egg chambers that had normal Gurken protein expression.
*P>0.05; **P<0.01. (E-H)
The increased HRP-Grk signal (green) in dorsal follicle cells (E) was
colocalized with CblL, which was ectopically expressed by EQ-Gal4 and
stained with 8C4 antibody (F, red). (H) The composite image of several
confocal sections of an egg chamber with an HRP-grk transgene and
CblL overexpression. (I-K) Some of these HRP-Grk signals were also
colocalized with Rab5 (I), Rab7 (J) and Egfr (K). Arrows indicate punctate
signals that overlapped in merged images. The bracket area is enlarged in
E',F',G',I',J',K'. Scale bars: 40 µm in
A-C; 20 µm in E-J; 10 µm in K.
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