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Fig. 3. Expression pattern and cell-autonomous function of brpf1 in
zebrafish CNC. Staining with reagents indicated at lower right at the
stages indicated upper right. Numbers of pharyngeal arches are indicated
(1-7). (A-J) Wild-type embryos; (K)
brpf1b943 mutant (-/-); (L-O) mutant transplanted
with wild-type cells (WT 
-/-). (A) Dorsal view; (B-E,J-O) lateral
views; (F-H) horizontal section; (I) longitudinal section. (A-D) At 26 hpf,
brpf1 is co-expressed with the CNC marker dlx2a (A) and with
fli1a (D), stained by anti-GFP immunostaining of
tg(fli1a:EGFP) embryo (Isogai et
al., 2003). brpf1-positive cells between CNC include
pharyngeal endoderm [D; compare with Fig.
1A in Crump et al. (Crump et al., 2004)]. (E-I) At 55 hpf,
sox9a-positive chondrocytes of cartilage condensates (cc; H)
(Yan et al., 2002) and
pax9a-positive pharyngeal endodermal cells (pe; G)
(Nornes et al., 1996;
Okabe and Graham, 2004) lack
brpf1 expression, which, however, is strongly expressed in p63
(tp63 - ZFIN)-positive cells
(Carney et al., 2007) of the
pharyngeal ectoderm (pec; F-H), in the oral ectoderm (oe; I) and in facial
ectoderm ventral to arches 1 and 2 (vfe; I)
(Crump et al., 2006). (J-O)
Analysis of chimeric embryos with rhodamine-dextran (RD)-labeled (N) and
tg(fli1a:EGFP)-positive (M) wild-type cells integrated in the CNC of
a brpf1 mutant host [for procedure, see Crump et al.
(Crump et al., 2006)]. Only
wild-type, not adjacent mutant CNC, cells display hoxa2b expression
(arrows in L and M; n=3/3). g, gut; op, opercle.
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